The CEnter of Study on AM Monexenics has developed an extensive know-how on the in vitro culture i.e. the monoxenic culture of arbuscular mycorrhizal fungi (AMF).

Symbiosis with fungi has been determinant for the evolution of vascular plants since their apparition on land. Devonian Rhynia fossils 400 million years old, permit to observe in the lower part of their stems, fungal structures closely resembling modern Glomales.

During this evolution, arbuscular mycorrhizal (AM) fungi became totally dependant on their host and became obligate symbionts. Today, in the present state of knowledge, it is impossible to grow these fungi independently from a host plant.

Since the mid-eighties, the use of root organ culture has opened new vistas on several aspects of the AM symbiosis. It has become obvious that all areas of the AM fungi biology per se, as well as of the biology of the symbiotic relationship, have been revisited using monoxenic cultures. Cultivation of AM fungi on root cultures have shed new lights on their molecular biology, cytology, genetic, physiology, systematic and phylogeny, which have since received a tremendous innovative momentum. Large scale industrial production of biologically clean AM inocula produced on root cultures has also become a reality.

With permission from Fortin, Declerck and Strullu, in In vitro culture of mycorrhizas, by Declerck S., Strullu D.G. and Fortin J.A., Springer-Verlag.

The CEnter of Study on AM Monoxenics of the Microbiology unit of the “Université catholique de Louvain” (Belgium) has made the in vitro cultivation of AMF its premium activity to elucidate various aspects, from genomic to functional, of the obligate biotrophic nature of these ubiquitous below-ground fungi.

HAM-P system

International training on in vitro culture of Arbuscular Mycorrhizal Fungi 2016

Dear colleagues, The CEnter of Study on AM Monoxenics (CESAMM) and the Glomeromycota IN vitro COllection (GINCO) have made the in vitro cultivation of AM fungi their primary research tool to study these fungi and their relationships with their host plants. Since 2005, CESAMM and GINCO organize an international training to offer the possibility to …

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To complete the promotion of the in vitro cultivation of AM fungi, we propose a specific training devoted to the process of AM fungi in vitro culture from the establishment to the maintenance following the methodologies routinely used in GINCO. The CESAMM offers also numerous opportunities to conduct thesis and trainings. Finally, a didactic server …

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