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The next training session is scheduled in June 10th-14th, 2024

Presentation of the international training on in vitro culture of arbuscular mycorrhizal fungi


The training staff of the UCLouvain Mycology Laboratory

The techniques for in vitro cultivation of AM fungi on excised roots (Declerck, Fortin and Strullu, 2005) and autotrophic plants (HAM-P and MD-P)(Voets et al., 2005; Voets et al., 2009) are used since many years at the CEntre of Study on AM Mononexics (CESAMM) as useful tools to investigate plant-fungus associations.

These systems can be used for a wide array of research topics. Indeed, they offer unique advantages in genetics, cell biology, biodiversity and physiological investigations of AM fungi and their hosts. Non-destructive microscopic observations, reliable cell physiology studies, clean biochemical and molecular analyses, highly controlled interaction studies with other micro-organisms and the potential to develop reliable in vitro mycorrhization of micro-propagated plants at an industrial scale, are amongst the applications permitted by these techniques. The proposed training will allow you to master both the root organ cultivation technique (i.e. the in vitro culture of an AM fungi with excised roots) and the autotrophic plant in vitro mycorrhization technique. We also offer a new session on the Ri T-DNA transformation of roots.

This training is addressed to scientists, technicians and industrials who aim at developing their research potential and the production of contaminant free AM inoculum.

Glomus etunicatum (MUCL 47650)

Claroideoglomus etunicatum (W.N. Becker & Gerd) C. Walker & A. Schüßler 2010 (MUCL 47650) in vitro culture


Declerck S., Strullu D.G and Fortin J.A. (2005). In vitro culture of mycorrhizas. Heidelberg, Springer-Verlag. 386 p.

Voets L., de la Providencia I.E., Fernandez K., IJdo M., Cranenbrouck S. and Declerck S. (2009). Extraradical mycelium network of arbuscular mycorrhizal fungi allows fast colonization of seedlings under in vitro conditions. Mycorrhiza 19 :347-356.

Voets, L., Dupré de Boulois, H., Renard, L., Strullu, D.G., Declerck, S. (2005). Development of an autotrophic culture system for the in vitro mycorrhization of potato plantlets. FEMS Microbiology Letters 248: 111-118.

General presentation of the UCLouvain Mycology Laboratory

Presentation of the training and interviews of the participants start at 4:35 min of the video

UCL Laboratory of Mycology BCCM-MUCL

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Author: Zayid Benyahia

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