The techniques for in vitro cultivation of AM
fungi on excised roots (Declerck, Fortin and
Strullu, 2005) and autotrophic plants (HAM-P and MD-P)(Voets et
al., 2005; Voets et al., 2009) are
used since many years at the CEntre of Study on
AM Mononexics (CESAMM) as useful tools to
investigate plant-fungus associations.
These systems can be used for a wide array of
research topics. Indeed, they offer unique
advantages in genetics, cell biology,
biodiversity and physiological investigations of
AM fungi and their hosts. Non-destructive
microscopic observations, reliable cell
physiology studies, clean biochemical and
molecular analyses, highly controlled interaction
studies with other micro-organisms and the
potential to develop reliable in vitro
mycorrhization of micro-propagated plants at an
industrial scale, are amongst the applications permitted by these techniques.
The proposed training will allow you to master
both the root organ cultivation technique (i.e.
the in vitro culture of an AM fungi with excised
roots) and the autotrophic plant in
vitro mycorrhization technique. We also offer a new
session on the Ri T-DNA transformation of roots.
This training is addressed to scientists,
technicians and industrials who aim at developing
their research potential and the production of contaminant free AM inoculum.
