The techniques for in vitro cultivation of AM
fungi on excised roots (Declerck, Fortin and
Strullu, 2005) and autotrophic plants (HAM-P and MD-P)(Voets et
al., 2005; Voets et al., 2009) are
used since many years at the CEntre of Study on
AM Mononexics (CESAMM) as useful tools to
investigate plant-fungus associations.
These systems can be used for a wide array of research topics. Indeed, they offer unique advantages in genetics, cell biology, biodiversity and physiological investigations of AM fungi and their hosts. Non-destructive microscopic observations, reliable cell physiology studies, clean biochemical and molecular analyses, highly controlled interaction studies with other micro-organisms and the potential to develop reliable in vitro mycorrhization of micro-propagated plants at an industrial scale, are amongst the applications permitted by these techniques. The proposed training will allow you to master both the root organ cultivation technique (i.e. the in vitro culture of an AM fungi with excised roots) and the autotrophic plant in vitro mycorrhization technique. We also offer a new session on the Ri T-DNA transformation of roots.
This training is addressed to scientists, technicians and industrials who aim at developing their research potential and the production of contaminant free AM inoculum.